货号:SBB-PS0028-C050 | 规格:50 ug | 目录价:¥4914 |
产品详情
产品名称:
SUMO1 (human) (rec.) (Rhodamine 110)
别名:
Small Ubiquitin-related Modifier 1; GAP-modifying Protein 1; SMT3C, SMT3H3, UBL1; Sentrin; Ubiquitin-homology Domain Protein PIC1
产品描述:
SUMO1 is a ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. This SUMO1 substrate is C-terminally derivatized with a bis-Gly-Rhodamine 110 fluorophore. The bis-Gly-Rh110 is quenched until the amide bond between the C-terminal glycine and the bis-Gly-Rh110 compound is hydrolyzed to mono-Gly-Rhodamine 110. The efficiency of quenching combined with the powerful signal upon hydrolysis yields a reagent with unparalleled signal-to-background. SUMO1-Rh110 can be used to study the deSUMOylating activity of hydrolases SENP1 and SENP2, or other deSUMOylating enzymes. The substrate activity of SUMO1-Rhodamine 110 was determined by measuring the SENP1 catalyzed release of unquenched mono-Gly-Rh110.
宿主来源:
E. coli
偶联:
Rhodamine
产品浓度:
Lot dependent.
保存温度:
-80°C
运输温度:
Dry Ice
预期分子量:
~12kDa
产品形式:
liquid
存储溶液:
Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride.
有效期:
Stable for at least 1 year after receipt when stored at -80°C.
产地:
瑞士
纯度:
>97% (LCMS)
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