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    ITCH E3 Ligase TR-FRET Kit
    货号：SBB-KF0035-KI01 规格：1 Kit 目录价：￥14168
    产品详情
    产品名称:
    ITCH E3 Ligase TR-FRET Kit
    别名:
    AIF4; AIP4; NAPP1
    产品描述:
    South Bay Bio’s ITCH TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto ITCH in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto ITCH in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
    靶标/特异性:
    Readout: Endpoint/Kinetic Label or Dye: Europium-Cryptate & Cy5 Detection Method: TR-FRET Substrate Properties: Protein-based Substrate. Typical experimental concentration is 1x. Excitation/Emission (nm): 304/620 & 304/665
    保存温度:
    -80°C
    运输温度:
    Dry Ice
    产地:
    瑞士
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