ICOS/CD278 Extracellular Domain (human, recombinant)

Activation-inducible Lymphocyte Immunomediatory Molecule|AILIM|CVID1|Inducible T Cell Costimulator

Inducible T cell co-stimulator (ICOS) is a glycoprotein and member of the CD28/B7 family of co-stimulatory receptors that promotes T cell activation.{57036} It exists as a membrane-bound disulfide-linked homodimer and contains a leader peptide, an extracellular domain that interacts with ICOS ligand (ICOSL), and a cytoplasmic tail that mediates association with PI3K and is critical for T cell activation.{57036,57037} ICOS is expressed on activated CD4+ and CD8+ T cells following T cell receptor crosslinking and/or co-stimulation by CD28.{57036} ICOS co-stimulation of T cells induces production of IL-4, IL-5, IL-6, IFN-γ (Item Nos. 32008 | 32078), TNF-α (Item Nos. 32020 | 32069), and GM-CSF (Item Nos. 32044 | 32079), but not IL-2, and superinduces production of IL-10, as well as prevents T cell apoptosis.{57037} Human melanoma cells express ICOSL and promote the expansion of ICOS+ IL-10-secreting regulatory T cells, which is positively correlated with unfavorable clinical outcomes.{57039} Administration of an anti-ICOS monoclonal antibody ameliorates mucosal inflammation and damage in a mouse model of T helper cell-induced chronic colitis.{57038} Cayman's ICOS/CD278 Extracellular Domain (human, recombinant) protein can be used for ELISA. This protein is a disulfide-linked homodimer. The reduced monomer, comprised of ICOS (amino acids 21-141) fused to His-tagged human IgG1 Fc at its C-terminus, consists of 386 amino acids, has a calculated molecular weight of 41.6 kDa, and a predicted N-terminus of Glu21 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at approximately 50 kDa by SDS-PAGE under reducing conditions.

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Lyophilized from sterile PBS, pH 7.4

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≥90% estimated by SDS-PAGE