Enzo Life Sciences的CYTO-ID®绿色/红色长期细胞示踪试剂盒采用专有的非共价细胞标记技术，将含有疏水性脂肪链的荧光染料稳定地结合入细胞膜的脂质双分子层中。标记缓冲液对哺乳动物细胞是等渗的，不含去污剂或有机溶剂。染料对细胞没有毒性，可在细胞中保留长达96小时，并在有丝分裂时传递给子细胞。由于该染料不对细胞内的蛋白质进行共价修饰，与基于硫醇反应的氯甲基或胺反应的琥珀酰亚胺酯类荧光染料的分子探针相比，细胞正常的生理反应得到更好的保留。

也可以使用CELLESTIAL®染料进行双重标记。标记的细胞可以通过共聚焦荧光显微镜等仪器进行观察。此外，染料标记的和未标记的细胞群可通过流式细胞仪进行分析。在延长96小时的培养后，没有观察到荧光转移到相邻细胞。这与Calcein AM和BCECF AM标记形成鲜明对比，后者在生理温度下只能在存活的细胞内保留几个小时。

Enzo Life Sciences的CYTO-ID®长期细胞示踪试剂盒适用于多种应用，如追踪细胞谱系，长期细胞活力、细胞毒性、细胞粘附、细胞迁移和细胞-细胞融合研究。

产品特点

● 可使用各种CELLESTIAL® 荧光探针进行双重标记

● 荧光从染料标记的细胞转移到未标记的细胞的情况极少

● 适用于长期细胞存活率、细胞毒性、细胞粘附、细胞迁移和细胞间融合测定

实验示例

图1.明场（A）和荧光显微镜图像（B）显示了用CYTO-ID® Green Tracer dye对Jurkat细胞的染色。使用标准的FITC（绿色）滤光片组对膜结合信号进行成像。

图2.流式细胞术分析Jurkat细胞混合群随时间变化的荧光。将用 CYTO-ID®绿色示踪染料染色的Jurkat细胞与未染色的Jurkat细胞群混合并孵育120小时。

图3.明场（A）和荧光显微镜图像（B）显示了用CYTO-ID® Red Tracer dye对Jurkat细胞的染色。使用标准的Texas Red（红色）滤光片组对膜结合信号进行成像。

图4.荧光显微镜图像显示了用CYTO-ID® Red Tracer dye对HeLa细胞的染色。使用标准的Texas Red（红色）滤光片组对膜结合信号进行成像(20x)。

图5.流式细胞术分析Jurkat细胞混合群随时间变化的荧光。将用 CYTO-ID®红色示踪染料染色的Jurkat细胞与未染色的Jurkat细胞群混合并孵育96小时。

产品信息

部分产品引用文献

1. Proangiogenic effects of tumor cells on endothelial progenitor cells vary with tumor type in an in vitro and in vivo rat model: R. An, et al.; FASEB J. 32, 5587 (2018), Application(s): Identification of TR-BME2 cells in tube-formation co-culture assay with CC521 colon adenocarcinoma cells

2. A liquid biopsy-based method for the detection and quantification of circulating tumor cells in surgical osteosarcoma patients: H. Zhang , et al.; Int. J. Oncol. 50, 1075 (2017), Application(s): Use with HOS cells (osteosarcoma). Microscopy

3. INBRX-120, a CD8α-targeted detuned IL-2 that selectively expands and activates tumoricidal effector cells for safe and durable in vivo responses: F. J. Sulzmaier, et al.; J. Immunother. Cancer 11, e006116 (2023)

4. Epigenetic modulation of immune synaptic-cytoskeletal networks potentiates γδ T cell-mediated cytotoxicity in lung cancer: R.R. Weng, et al.; Nat. Commun. 12, 2163 (2021)

5. Characterization and Cellular Internalization of Spherical Cellulose Nanocrystals (CNC) into Normal and Cancerous Fibroblasts: N.A.H. Shazali, et al.; Materials (Basel) 12, 3251 (2019), Application(s): C6 and NIH3T3 cell tracing

6. Time resolved 3D live-cell imaging on implants: A. Ingendoh-Tsakmakidis, et al.; PLoS One 13, e0205411 (2018)

7. Nerve guidance conduit with a hybrid structure of a PLGA microfibrousbundle wrapped in a micro/nanostructured membrane: S.W. Peng, et al.; Int. J. Nanomedicine 12, 421 (2017), Application(s):Tracking in vitro cells migration (immortalized neuron progenitor KT 98)

8. Thioreductase-containing epitopes inhibit the development of type 1 diabetes in the NOD mouse model: E. Malek Abrahimians, et al.; Front. Immunol. 7, 67 (2016), Application(s): Flow cytometry analysis using isolated mouse spleen B cells

9. Syngeneic murine ovarian cancer model reveals that ascites enriches for ovarian cancer stem-like cells expressing membrane GRP78: L. Mo, et al.; Mol. Cancer Ther. 14, 747 (2015) 

10. An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture: Y. Takeshita, et al.; J. Neurosci. Methods 232, 165 (2014), Application(s): Confocal microscopy using adult human astrocyte cells

11. Vascular endothelial growth factor-C modulates proliferation and chemoresistance in acute myeloid leukemic cells through an endothelin-1-dependent induction of cyclooxygenase-2: K.T. Hua, et al.; Biochim. Biophys. Acta 1843, 387 (2014)

12. Expanding the Ig superfamily code for laminar specificity in retina: expression and role of contactins: M. Yamagata, et al.; J. Neurosci. 32, 14402 (2012)

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