Enzo Life Sciences公司的ER-ID® Green assay kit适用于活细胞或破膜醛固定细胞的染色。微摩尔级浓度的ER-ID®绿色染料足以对哺乳动物细胞进行染色，在人宫颈癌细胞系、T淋巴细胞系、Jurkat 细胞系、HeLa 细胞系以及骨肉瘤上皮细胞U2-OS中已经得到了验证。ER-ID® Green assay kit专门用于表达RFP或OFP的细胞系，以及表达蓝色或青色荧光蛋白（BFP、CFP）的细胞。该试剂盒适用于活细胞或固定后细胞与探针（如标记抗体）或与Texas Red或香豆素类似光谱性质的其他荧光缀合物结合使用。

产品特点

● 新型内质网染料，可用于活细胞、破膜细胞或固定细胞的染色

● 与常见荧光染料和荧光蛋白兼容

● 高耐光漂白性、抗浓度淬灭和光转换

● 生产严格，以控制和消除非特异性结果

 

实例分析

● ER-ID®绿色染色与含有KDEL序列的钙网蛋白共定位，与橙色荧光蛋白（OFP）融合。

 

● 用ER-ID®绿色染料（A）、Hoechst 33342染料（B）对HeLa活细胞进行染色，得到的合成图像（C）。

 

● 用4%多聚甲醛固定HepG2细胞20分钟，并用ER-ID®标记30分钟。下图为标记3天后拍摄的照片。

 

● 将HepG2细胞与ER-ID®孵育30分钟，并用倒置显微镜（Zeiss）进行分析。

 

产品详情：

产品名称	ER-ID® Green   assay kit
产品货号	ENZ-51025-K500
产品规格	500 assays
质量控制	A sample   from each lot of ER-ID® Green assay kit is used to stain HeLa cells, using   the procedures described in the user manual. The selectivity of the ER-ID®   Green dye is evident.
应用	Fluorescence   microscopy Enzo Life   Sciences’ ER-ID® Green assay kit contains a novel endoplasmic   reticulum-selective dye suitable for live cell, or detergent-permeabilized   aldehyde-fixed cell staining.
长期储存	-20°C
组分	ER-ID® Green   detection reagent: 50μl Hoechst   33342 nuclear stain: 50μl 10X assay   buffer: 15ml
产品说明	The ER-ID®   Green assay kit is a member of the CELLESTIAL® product line, reagents and   assay kits comprising fluorescent molecular probes that have been extensively   benchmarked for live cell analysis applications. CELLESTIAL® reagents

部分产品文献引用

1. Molecular interplay between NOX1 and autophagy in cadmium-induced prostate carcinogenesis: A. Tyagi, et al.; Free Radic. Biol. Med. 199, 44 (2023) 

2. Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocations into the endoplasmic reticulum: A. Komatsu, et al.; J. Biol. Chem. 298, 101933 (2022) 

3. PLP1 mutations in patients with multiple sclerosis: identification of a new mutation and potential pathogenicity of the mutations: N.C. Cloake, et al.; J. Clin. Med. 7, 342 (2018), Application(s): Fluorescence microscopy 

4. Englerin A induces an acute inflammatory response and reveals lipid metabolism and ER stress as targetable vulnerabilities in renal cell carcinoma: A. Batova, et al.; PLoS One 12, e0172632 (2017), Application(s): Fluorescence microscopy

5. Cell growth on (“Janus”) density gradients of bifunctional zeolite L crystals: N.S. Kehr, et al.; ACS Appl. Mater. Interfaces 8, 35081 (2016)

6. The parathyroid hormone second receptor PTH2R and its ligand tuberoinfundibular peptide of 39 residues TIP39 regulate intracellular calcium and influence keratinocyte differentiation: E. Sato, et al.; J. Invest. Dermatol. 136, 1449 (2016)

7. Viral genome imaging of hepatitis C virus to probe heterogeneous viral infection and responses to antiviral therapies: V. Ramanan, et al.; Virology 494, 236 (2016), Application(s): Endoplasmic reticulum staining

8. Localization-Dependent Cell-Killing Effects of Protoporphyrin (PPIX)-Lipid Micelles and Liposomes in Photodynamic Therapy: S. Tachikawa, et al.; Bioorg. Med. Chem. 23, 7578 (2015), Application(s): Cell staining

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