产品货号

CP131

产品规格

1/5/25/50/100mg

反应种属

Mouse

克隆号

AFS98-CP131

同种型

Mouse   IgG2a(switched from rat IgG2a)

免疫原

Not   available or unknown

实验应用

in   vivo macrophage depletion*
  in   vitro CSF1R neutralization*
  in vivo monocyte   depletion*
  Flow   cytometry*
  Western   blot*
  *Reported   for the original rat IgG2a AFS98 antibody

产品形式

PBS, pH   7.0,Contains no stabilizers or preservatives

纯度

>95%,   Determined by SDS-PAGE

聚合

<5%,   Determined by SEC

无菌处理

0.2 µm filtration

纯化方式

Protein A

分子量

150 kDa

小鼠病原检测

Ectromelia/Mousepox   Virus: Negative

Hantavirus:   Negative

K Virus:   Negative

Lactate   Dehydrogenase-Elevating Virus: Negative

Lymphocytic   Choriomeningitis virus: Negative

Mouse   Adenovirus: Negative

Mouse Cytomegalovirus:   Negative

Mouse   Hepatitis Virus: Negative

Mouse Minute   Virus: Negative

Mouse   Norovirus: Negative

Mouse   Parvovirus: Negative

Mouse   Rotavirus: Negative

Mycoplasma   Pulmonis: Negative

Pneumonia   Virus of Mice: Negative

Polyoma   Virus: Negative

Reovirus   Screen: Negative

Sendai   Virus: Negative

Theiler’s   Murine Encephalomyelitis: Negative

保存条件

抗体原液保存在4°C，不能冷冻保存。

推荐同型对照

InVivoPlus   mouse IgG2a isotype control, unknown specificity（货号BP0085）

推荐抗体稀释液

InVivoPure   pH 7.0 Dilution Buffer（货号IP0070）

体内巨噬细胞耗竭

(in vivo macrophage 

depletion)

1. Bauche, D., et al. (2018). 'LAG3(+) Regulatory   T Cells Restrain Interleukin-23-Producing 

CX3CR1(+) Gut-Resident Macrophages during Group 3   Innate Lymphoid Cell-Driven Colitis' 

Immunity 49(2): 342-352 e345.

2. Gordon, S. R., et al. (2017). 'PD-1 expression   by tumour-associated macrophages inhibits 

phagocytosis and tumour immunity' Nature 545(7655):   495-499.

3. Moynihan, K. D., et al. (2016). 'Eradication of   large established tumors in mice by combination 

immunotherapy that engages innate and adaptive immune   responses' Nat Med. doi : 10.1038/nm.4200.

4. Naik, S., et al. (2015).   'Commensal-dendritic-cell interaction specifies a unique protective 

skin immune signature' Nature 520(7545): 104-108.

体外CSF-R1中和

(in vitro CSF-R1 

neutralization)

1.Sheng, K. C., et al. (2014). 'IL-3 and CSF-1 interact   to promote generation of 

CD11c+IL-10-producing macrophages' PLoS One 9(4):   e95208. 

流式细胞术

(Flow   Cytometry)

1. Li, W., et al. (2012). 'Intravital 2-photon   imaging of leukocyte trafficking in beating 

heart' J Clin Invest 122(7): 2499-2508. 

2. Tagliani, E., et al. (2011). 'Coordinate   regulation of tissue macrophage and dendritic 

cell population dynamics by CSF-1' J Exp Med 208(9):   1901-1916.