产品货号

CP129

产品规格

1/5/25/50/100mg

反应种属

Mouse

克隆号

1A8-CP129

同种型

Mouse   IgG2a(switched from rat IgG2a)

免疫原

EL4J cells   transfected with Ly6G

实验应用

in vivo   neutrophil depletion*

in vivo MDSC   depletion*

Immunofluorescence*

Immunohistochemistry   (paraffin)*

Immunohistochemistry   (frozen)*

Flow   cytometry*

*Reported   for the original rat IgG2a 1A8 antibody

产品形式

PBS, pH   7.0,Contains no stabilizers or preservatives

纯度

>95%,   Determined by SDS-PAGE

聚合

<5%, Determined   by SEC

无菌处理

0.2 µm   filtration

纯化方式

Protein G

分子量

150 kDa

小鼠病原检测

Ectromelia/Mousepox   Virus: Negative

Hantavirus:   Negative

K Virus:   Negative

Lactate   Dehydrogenase-Elevating Virus: Negative

Lymphocytic   Choriomeningitis virus: Negative

Mouse   Adenovirus: Negative

Mouse   Cytomegalovirus: Negative

Mouse   Hepatitis Virus: Negative

Mouse Minute   Virus: Negative

Mouse   Norovirus: Negative

Mouse   Parvovirus: Negative

Mouse   Rotavirus: Negative

Mycoplasma   Pulmonis: Negative

Pneumonia   Virus of Mice: Negative

Polyoma   Virus: Negative

Reovirus   Screen: Negative

Sendai   Virus: Negative

Theiler’s   Murine Encephalomyelitis: Negative

保存条件

抗体原液保存在4°C，不能冷冻保存。

推荐同型对照

InVivoPlus   mouse IgG2a isotype control, unknown specificity（货号BP0085）

推荐抗体稀释液

InVivoPure   pH 7.0 Dilution Buffer（货号IP0070）

体内中性粒细胞耗竭

(in   vivo neutrophil depletion)

1. Davis, R. W. t., et al. (2018). 'Luminol Chemiluminescence   Reports Photodynamic 

Therapy-Generated Neutrophil Activity In Vivo and   Serves as a Biomarker of Therapeutic 

Efficacy' Photochem Photobiol .

2. Moynihan, K. D., et al. (2016). 'Eradication of   large established tumors in mice by 

combination immunotherapy that engages innate and   adaptive immune responses' Nat Med. 

doi : 10.1038/nm.4200.

3. Conde, P., et al. (2015). 'DC-SIGN(+)   Macrophages Control the Induction of Transplantation 

Tolerance' Immunity 42(6): 1143-1158.

4. Griseri, T., et al. (2015). 'Granulocyte   Macrophage Colony-Stimulating Factor-Activated 

Eosinophils Promote Interleukin-23 Driven Chronic   Colitis' Immunity 43(1): 187-199.

5. Yamada, D. H., et al. (2015). 'Suppression of   Fcgamma-receptor-mediated antibody 

effector function during persistent viral infection'   Immunity 42(2): 379-390. 

体内中性粒细胞耗竭、流式细胞术、

免疫组化石蜡切片(in vivo   neutrophil 

depletion, Flow Cytometry, 

Immunohistochemistry (paraffin))

Coffelt, S. B., et al. (2015). 'IL-17-producing   gammadelta T cells and neutrophils conspire 

to promote breast cancer metastasis' Nature 522(7556):   345-348.

体内中性粒细胞耗竭、流式细胞术、

免疫组化石蜡切片、免疫组化冰冻切片

(in vivo neutrophil depletion, Flow 

Cytometry, Immunohistochemistry 

(paraffin), Immunohistochemistry (frozen)

Finisguerra, V., et al. (2015). 'MET is required for   the recruitment of anti-tumoural 

neutrophils' Nature 522(7556): 349-353.

体内中性粒细胞耗竭、流式细胞术

(in vivo neutrophil depletion, 

Flow Cytometry)

1.Moser, E. K., et al. (2014). 'Late engagement of CD86   after influenza virus 

clearance promotes recovery in a FoxP3+ regulatory T   cell dependent manner' 

PLoS Pathog 10(8): e1004315.

2. Chen, K. W., et al. (2014). 'The neutrophil NLRC4   inflammasome selectively 

promotes IL-1beta maturation without pyroptosis during   acute Salmonella 

challenge' Cell Rep 8(2): 570-582.

3. Garraud, K., et al. (2012). 'Differential role of   the interleukin-17 axis and 

neutrophils in resolution of inhalational anthrax'   Infect Immun 80(1): 131-142.

体内骨髓来源抑制细胞耗竭

(in vivo MDSC depletion)

Deng, L., et al. (2014). 'Irradiation and anti-PD-L1   treatment synergistically 

promote antitumor immunity in mice' J Clin Invest   124(2): 687-695.

体内中性粒细胞耗竭、

免疫荧光(in   vivo neutrophil 

depletion, Immunofluorescence)

Edelson, B. T., et al. (2011). 'CD8alpha(+) dendritic   cells are an obligate 

cellular entry point for productive infection by   Listeria monocytogenes' 

Immunity 35(2): 236-248.