EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher生产的重组单核小体 （H3K4me3） 由 147 个DNA碱基对缠绕在组蛋白的八聚体核心（H2A、H2B、H3.2 和 H4 各两个）上构成，是染色质的基本单位。Lowary和Widom[1]鉴定的147bp 601序列对组蛋白八聚体具有高亲和力，可用于核小体组装。H3K4me3 dNuc 在组蛋白H3.2的第4位含有三甲基赖氨酸。H3K4me3在110位处有一个Cys到Ala的取代。该DNA含有一个5’biotin-TEG基团。EpiCypher生产的重组单核小体适用于多种应用，包括用作酶分析、高通量筛选和抑制剂测试、染色质结合研究、蛋白质-蛋白质相互作用分析、结构研究和效应蛋白结合实验中的底物。

[1] Lowary and Widom J. Mol. Biol. (1998). PMID: 9514715

产品详情

宿主来源: Human

表达系统: E. coli & synthetic DNA

标签: Biotinylated

保存温度: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws

产品形式: 0.977 mg/mL mononucleosome in 51.2 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol (27.3 µg protein, 50 µg DNA + protein)

验证数据

	Figure 1: Western blot data Western Analysis of H3K4me3 dNuc. Top Panel: Unmodified (EpiCypher 16-0006;  Lane 1) and H3K4me3 (Lane 2) nucleosomes were probed with an anti-H3K4me3  antibody and analyzed via ECL readout. Only the H3K4me3 sample produced a  detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified  (Lane 1) and H3K4me3 (Lane 2) nucleosomes.
Figure 2: Mass spec data Synthetic H3K4me3 histone analyzed by high resolution mass spectrometry.  Expected mass = 15,267.77 Da. Determined mass = 15,266.15 Da.
	Figure 3: Protein gel data Coomassie stained SDS-PAGE gel of proteins in H3K4me3 dNuc (1 µg) demonstrates  the purity of histones in the preparation. Sizes of molecular weight markers and positions  of the core histones (H2A, H2B, H3K4me3 and H4) are indicated.
	Figure 4: DNA gel data H3K4me3 dNuc resolved via native PAGE gel and stained with ethidium bromide to  visualize DNA. Lane 1: Free DNA (EpiCypher 18-0005; 100 ng). Lane 2: Intact  H3K4me3 nucleosomes (400 ng).

订购详情

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