蛋白质羰基化是蛋白质氧化损伤的一种，是氧化应激中一种不可逆的化学修饰。蛋白质羰基化会引起蛋白质结构的改变，使其失去原有的生物学功能，最终导致细胞和组织功能紊乱。蛋白质羰基化不仅是氧化损伤的一项重要指标，也参与多种疾病的生理和病理过程，比如衰老，凋亡，神经退行性疾病等。蛋白质样品中蛋白质羰基化的量可以用二硝基苯肼(DNP)衍生化并用比色法或免疫法检测结合的DNP来测定。ELISA方法能够用微克蛋白质定量测检测羰基化合物。
Enzo Life Sciences提供的Protein carbonyl ELISA kit是一种于抗体的检测试剂盒。与比色分析相比，它具有更高的灵敏度、更低的背景、可检测更多的样本。
产品特点
◆ 高灵敏度ELISA，可检测低水平的羰基化蛋白
◆ 已在多种类型样本中验证
◆ 超越半定量Western Blot分析的全定量检测
产品信息
产品货号 | ALX-850-312-KI01 |
产品名称 | Protein carbonyl ELISA kit |
规格 | 96 wells |
检测时间 | 4.5 hours |
适用样本类型 | 血浆，其他体液，细胞和组织提取物 |
适用种属 | 不限种属 |
检测波长 | 450 nm |
标准曲线示例
部分产品文献引用
1. Cardiovascular and Blood Oxidative Stress Responses to Exercise and Acute Woodsmoke Exposure in Recreationally Active Individuals: C.M. Williamson-Reisdorph, et al.; Wilderness Environ. Med. 33, 17-31 (2022)
2. Preliminary experience on the use of sucrosomial iron in hemodialysis: focus on safety, hemoglobin maintenance and oxidative stress: F. Reggiani, et al.; Int. Urol. Nephrol. 10, 11255 (2021)
3. A longitudinal study of neurotrophic, oxidative, and inflammatory markers in first-onset depression in midlife women: M.A. Pasquali, et al.; Eur. Arch. Psychiatry Clin. Neurosci. 268, 771 (2018), Application(s): ELISA using human serum
4. Antibiotic bedaquiline effectively targets growth, survival and tumor angiogenesis of lung cancer through suppressing energy metabolism: X. Wu, et al.; Biochem. Biophys. Res. Commun. 495, 267 (2018), Application(s): Human lung cancer cells
5. Antibiotic drug piperacillin induces neuron cell death through mitochondrial dysfunction and oxidative damage: S. Jiang, et al.; Can. J. Physiol. Pharmacol. 96, 562 (2018), Application(s): SH-SY5Y cell lysates
6. Plasma protein carbonylation in haemodialysed patients: focus on diabetes and gender: G. Colombo, et al.; Oxid. Med. Cell. Longev. 2018, 4149681 (2018), Application(s): ELISA using human plasma
7. The biological age linked to oxidative stress modifies breast cancer aggressiveness: M.D.M. Saez-Freire, et al.; Free Radic. Biol. Med. 120, 133 (2018), Application(s): ELISA using liver homogenates
8. Tigecycline as a dual inhibitor of retinoblastoma and angiogenesis via inducing mitochondrial dysfunctions and oxidative damage: Y. Xiong, et al.; Sci. Rep. 8, 11747 (2018)
9. Antibiotic tigecycline enhances cisplatin activity against human hepatocellular carcinoma through inducing mitochondrial dysfunction and oxidative damage: J. Tan, et al.; Biochem. Biophys. Res. Commun. 483, 17 (2017), Application(s): HCC cell lysates
10. Induction of Mitochondrial Dysfunction and Oxidative Damage by Antibiotic Drug Doxycycline Enhances the Responsiveness of Glioblastoma to Chemotherapy: Q. Tan, et al.; Med. Sci. Monit. 23, 4117 (2017), Application(s): Human glioblastoma cell lines A172 and U87 lysates
11. Targeting mitochondrial respiration selectively sensitizes pediatric acute lymphoblastic leukemia cell lines and patient samples to standard chemotherapy: X. Fu, et al.; Am. J. Cancer Res. 7, 2395 (2017)
12. Impact of chromium dinicocysteinate supplementation on inflammation, oxidative stress, and insulin resistance in type 2 diabetic subjects: an exploratory analysis of a randomized, double-blind, placebo-controlled study: Z.M. Saiyed, et al.; Food Nutr. Res. 60, 31762 (2016), Application(s): Measurement of biomarkers in plasma
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