产品货号

BE0015-1

产品规格

1/5/25/50/100mg

反应种属

Mouse

克隆号

37.51

同种型

Syrian Hamster IgG2

免疫原

C57BL/6 mouse T cell lymphoma EL-4 cells

实验应用

in vitro T cell stimulation/activation

in vivo CD28 blockade

产品形式

PBS, pH 6.0 0.01% Tween,Contains no stabilizers or preservatives

纯度

>95%, Determined by SDS-PAGE

无菌处理

0.2 µm filtration

纯化方式

Protein G

RRID

AB_1107624

分子量

150 kDa

保存条件

抗体原液保存在4°C，不能冷冻保存。

推荐同型对照

InVivoMAb polyclonal Syrian hamster IgG（货号BE0087）

推荐抗体稀释液

InVivoPure pH 6.0T Dilution Buffer（货号IPT060）

体外T细胞刺激/激活

(in vitro T cell stimulation/activation)

1. Lacher, S. M., et al. (2018). 'NF-kappaB inducing kinase (NIK) is an essential post-transcriptional regulator of T-cell activation affecting F-actin dynamics and TCR signaling' J Autoimmun 94: 110-121.

2. Wendland, K., et al. (2018). 'Retinoic Acid Signaling in Thymic Epithelial Cells Regulates Thymopoiesis' J Immunol 201(2): 524-532.

3. Ron-Harel, N., et al. (2016). 'Mitochondrial Biogenesis and Proteome Remodeling Promote One-Carbon Metabolism for T Cell Activation' Cell Metab 24(1): 104-117.

4. Gu, A. D., et al. (2015). 'A critical role for transcription factor Smad4 in T cell function that is independent of transforming growth factor beta receptor signaling' Immunity 42(1): 68-79.

5. Choi, Y. S., et al. (2015). 'LEF-1 and TCF-1 orchestrate TFH differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6' Nat Immunol 16(9): 980-990.  

体内CD28阻断

(in vivo CD28 blockade)

1. Rouhani, S. J., et al. (2015). 'Roles of lymphatic endothelial cells expressing peripheral tissue antigens in CD4 T-cell tolerance induction' Nat Commun 6: 6771.

2. Eberlein, J., et al. (2012). 'Multiple layers of CD80/86-dependent costimulatory activity regulate primary, memory, and secondary lymphocytic choriomeningitis virus-specific T cell immunity' J Virol 86(4): 1955-1970.