品牌：Mirus

货号：MIR 2300

品名：TransIT®-LT1 Transfection Reagent

产品信息：

产品类型：转染试剂

规格：1ml

应用：稳定转染；病毒生产；干细胞转染。

储存条件：4℃

保质期：购买产品起1年。

产品介绍：

TransIT®-LT1 Transfection Reagent可用于广谱DNA递送-对多种细胞使用一种转染试剂和协议；低细胞毒性-维持细胞密度，减少实验偏差；高效传递-在大量细胞中实现表达，以获得实验成功；提供单粒或多粒质粒-适用于病毒生产等应用。

Exceptional Transfection Efficiency in Human Induced Pluripotent Stem Cells (iPSCs) via Reverse Transfection with TransIT®-LT1. The TransIT®-LT1 Transfection Reagent was used to reverse transfect 1.3 x 106 iPS cells with a ZsGreen expressing plasmid (Clontech). Reverse transfections were performed in 6-well plates using 12 µl of TransIT®-LT1 Transfection Reagent to deliver 4 µg of DNA (3:1, reagent: DNA). Cells were visualized 48 hours post-transfection and imaged under a 10X objective with an Olympus IX71® Inverted Microscope. Images are (A) phase contrast and (B) green fluorescence. Cells were assayed 48 hours post-transfection on an Accuri® Cytometer. The histogram (C) shows untransfected cells (black line) compared to cells transfected with plasmid using TransIT®-LT1 (green line). 

High Efficiency Transfection of iCell® Cardiomyocytes Using TransIT®-LT1 Transfection Reagent. iCell® Cardiomyocytes were plated at 20,000 cells/well in a 96 well tissue culture plate coated with 0.1% gelatin. After allowing the cells to recover from thaw, cells were transfected with 100 ng/well of pMAXGFP (Lonza) using TransIT®-LT1 Transfection Reagent with a 2:1 reagent-to-DNA ratio according to the manufacturer’s instructions. Fluorescent images were taken 3 days post transfection using a Olympus IX71® inverted microscope. 

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