●Specific detection of Fe2+ 

● 特异性检测Fe2+ 

●Applicable to live cell imaging of labile Fe2+ 

● 适用于不稳定的Fe2+的活细胞成像 

●For detection of Fe2+ in aqueous media 

● 适合于检测水溶性基质里的Fe2+ 

●For detection of labile iron in living cells 

● 适合于检测活细胞内不稳定的离子 

enceappears aroResponse of FeRhoNox™-1 to Fe2+. Reacting FeRhoNox™-1 with Fe2+for 1 h at 37℃, peak of the fluorescund 575 nm.

Fe2+detection by FeRhoNox™-1in HepG2 cells. HepG2 cells preincubated with/without Fe(NH4)2(SO4)2(FAS: 100 µM) for 0.5 hrwas incubated with 5 µM of FeRhoNox™-1 for 1 hr.

The selectivity of FeRhoNox™-1.FeRhoNox™-1 can react with only Fe2+selectively.

Comparison between FeRhoNox™-1’s and Golgi Marker’s staining images. The Merge image shows that FeRhoNox™-1 selectively reacts with Fe2+in Golgi apparatus.

GC 901

FeRhoNox™-1

Fe2+in aqueous media, labile Fe2+in living cells

50μg×10

① - Fe2+ : not added Fe2+ （100 μM Ferrous ammoniumsulfate）

Bpy: cell permeable
Fe2+ selective chelator
(2,20-bipyridyl).

② +Fe2+ : added Fe2+

③ +Fe2+ +Bpy : added Fe2+ and Bpy(1 mM) as Fe2+ chelator

② shows increasing of FeRhoNox™-1’s fluorescence intensity and

③showsdecreasing of it. These results indicate that FeRhoNox™-1 reacts with Fe2+ selectively.

①-Fe2+: not added Fe2+ （100 μMFerrous ammonium sulfate）

②+Fe2+              : added Fe2+

③+Fe2+ +Bpy: added Fe2+and Bpy(1 mM) as Fe2+ chelator

②shows increasing of FeRhoNox™-1’s fluorescence intensity and

③shows decreasing of it. These results indicate that FeRhoNox™-1 
reacts with Fe2+ selectively.