Notch1 (mouse, recombinant; aa 19-526)

Motch A|Neurogenic Locus Notch Homolog Protein 1|Notch Receptor 1|Tan1

Notch1 is a single-pass transmembrane receptor and member of the Notch family.{62466} It is synthesized as a precursor that is cleaved by a furin-like convertase to produce two subunits: the noncovalently associated extracellular (NEC) subunit, which contains 36 EGF-like repeats involved in ligand binding, and the transmembrane (NTM) subunit, which contains the transmembrane domain and several other domains that, together, act as a ligand-activated transcription factor.{62466,62467,62468} Notch1 is expressed in endothelial cells, primarily in arteries, as well as in epididymal and subcutaneous fat and the brain.{62469,62470} It is bound by ligands from the Jagged or Delta families expressed on adjacent cells, which induces a conformational change and sequential cleavage by a disintegrin and metalloproteinase domain-containing protein (ADAM) and γ-secretase, releasing the intracellular domain, which translocates to the nucleus and induces the transcription of Notch1 target genes as part of a transcriptional activation complex.{62468} Notch1 is necessary for arterial-venous differentiation during development and the maintenance of hippocampal stem cells in adulthood.{62469,62471} It is also involved in tumorigenesis, regulating cell adhesion and metastasis, and is associated with a variety of solid tumor and hematological cancers.{62472,62473} Mutations in NOTCH1 have been found in patients with chronic lymphocytic leukemia (CLL) and T cell acute lymphoblastic leukemia (T-ALL) and are associated with poor outcomes in patients with CLL.{62468} Cayman’s Notch1 (mouse, recombinant; aa 19-526) protein can be used for binding assays. This protein consists of 518 amino acids, has a calculated molecular weight of 55 kDa, and a predicted N-terminus of Ala-19 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 80 kDa due to glycosylation.

-80

-80

Lyophilized from sterile 20 mM Tris, pH 7.4, with 500 mM sodium chloride and 10% glycerol

365

≥82% estimated by SDS-PAGE