产品货号

CP001

产品规格

1mg

反应种属

Mouse

克隆号

10F.9G2™-CP001

同种型

Mouse   IgG1(switched from rat IgG2b)

免疫原

Mouse CD274

实验应用

in vivo   PD-L1 blockade*

Immunofluorescence*

Immunohistochemistry   (frozen)*

Flow   cytometry*

Western   blot*

*Reported   for the original rat IgG2b 10F.9G2 antibody

产品形式

PBS, pH   7.0,Contains no stabilizers or preservatives

纯度

>95%,   Determined by SDS-PAGE

聚合

<5%,   Determined by SEC

无菌处理

0.2 µm   filtration

纯化方式

Protein A

分子量

150 kDa

小鼠病原检测

Ectromelia/Mousepox   Virus: Negative

Hantavirus: Negative

K Virus:   Negative

Lactate   Dehydrogenase-Elevating Virus: Negative

Lymphocytic   Choriomeningitis virus: Negative

Mouse   Adenovirus: Negative

Mouse   Cytomegalovirus: Negative

Mouse   Hepatitis Virus: Negative

Mouse Minute   Virus: Negative

Mouse   Norovirus: Negative

Mouse   Parvovirus: Negative

Mouse   Rotavirus: Negative

Mycoplasma   Pulmonis: Negative

Pneumonia   Virus of Mice: Negative

Polyoma   Virus: Negative

Reovirus   Screen: Negative

Sendai   Virus: Negative

Theiler’s   Murine Encephalomyelitis: Negative

保存条件

抗体原液保存在4°C，不能冷冻保存。

推荐抗体稀释液

InVivoPure   pH 7.0 Dilution Buffer（货号IP0070）

体内PD-L1阻断

(in vivo   PD-L1 blockade)

1. Grasselly, C., et al. (2018). 'The Antitumor   Activity of Combinations of Cytotoxic 

Chemotherapy and Immune Checkpoint Inhibitors Is   Model-Dependent' Front 

Immunol 9: 2100.

2. Stathopoulou, C., et al. (2018). 'PD-1   Inhibitory Receptor Downregulates Asparaginyl 

Endopeptidase and Maintains Foxp3 Transcription Factor   Stability in Induced Regulatory 

T Cells' Immunity 49(2): 247-263 e247.

3. Jaworska, K., et al. (2015). 'Both PD-1 ligands   protect the kidney from ischemia 

reperfusion injury' J Immunol 194(1): 325-333.

4. Kim, J., et al. (2015). 'Memory programming in   CD8(+) T-cell differentiation is 

intrinsic and is not determined by CD4 help' Nat Commun   6: 7994.

5. Zander, R. A., et al. (2015). 'PD-1   Co-inhibitory and OX40 Co-stimulatory Crosstalk 

Regulates Helper T Cell Differentiation and   Anti-Plasmodium Humoral Immunity' Cell 

Host Microbe 17(5): 628-641.

体内PD-L1阻断，流式细胞术

(in vivo PD-L1 blockade, Flow 

Cytometry)

1. Aloulou, M., et al. (2016). 'Follicular   regulatory T cells can be specific for the 

immunizing antigen and derive from naive T cells' Nat   Commun 7: 10579.

2. Ngiow, S. F., et al. (2015). 'A Threshold Level   of Intratumor CD8+ T-cell PD1 

Expression Dictates Therapeutic Response to Anti-PD1'   Cancer Res 75(18): 3800-3811.

3. Rutigliano, J. A., et al. (2014). 'Highly   pathological influenza A virus infection is 

associated with augmented expression of PD-1 by   functionally compromised 

virus-specific CD8+ T cells' J Virol 88(3): 1636-1651.

体内PD-L1阻断，免疫荧光

(in vivo PD-L1 blockade, 

Immunofluorescence)

1.Willimsky, G., et al. (2013). 'Virus-induced   hepatocellular carcinomas cause 

antigen-specific local tolerance' J Clin Invest 123(3):   1032-1043.

免疫组织化学（冷冻），免疫荧光

(Immunohistochemistry (frozen), 

Immunofluorescence)

1.Riella, L. V., et al. (2011). 'Essential role of PDL1   expression on nonhematopoietic 

donor cells in acquired tolerance to vascularized   cardiac allografts' Am J Transplant 

11(4): 832-840.